abs against stat3 antibody  (Abmart Inc)

Journal: Cells

Article Title: Repositioning Mifepristone as a Leukaemia Inhibitory Factor Receptor Antagonist for the Treatment of Pancreatic Adenocarcinoma.

doi: 10.3390/cells11213482

Figure Lengend Snippet: Figure 5. Mifepristone antagonizes LIFR and reduces STAT3 activation. (A) Mifepristone inhibition activity of LIFR/LIF binding accessed by a cell-free AlphaScreen assay. (B) STAT3 transactivation on HepG2 cells. Results are expressed as mean ± SEM of five samples per group.

Article Snippet: Protein extracts were electrophoresed on 12% acrylamide Tris-Glycine gel (Invitrogen), blotted to the nitrocellulose membrane, and then incubated overnight with primary Abs against STAT3 (sc-8019 1:500; Santa Cruz Biotechnology) and phosho-Stat3 (GTX118000 1:1000; Genetex).

Techniques: Activation Assay, Inhibition, Activity Assay, Binding Assay, Amplified Luminescent Proximity Homogenous Assay

Journal: Cells

Article Title: Repositioning Mifepristone as a Leukaemia Inhibitory Factor Receptor Antagonist for the Treatment of Pancreatic Adenocarcinoma.

doi: 10.3390/cells11213482

Figure Lengend Snippet: Figure 7. Mifepristone inhibits in vitro migration in STAT3-dependent signalling. (A) Relative mRNA expression of Vimentin and CXCR4. (B) Immunofluorescence analysis of Vimentin expression. (C) Scratch wound healing assay. MIA PaCa-2 cell monolayers were scraped in a straight line using a p200 pipette tip; then, they were left untreated or primed with LIF 10 ng/mL alone or in combination with mifepristone 10 or 20 µM and EC359 25 nM. The wound generated was captured at 0 and 48 h of incubation with the compounds above described. The images show cell migration at the two times point indicated. (D) Images of obtained points were analysed, measuring scraped area and its closure vs. the first time point at 0 h. Results are the mean ± SEM of three samples per group (* represents statistical significance versus NT, and # versus LIF, p < 0.05). (E) Analysis of STAT3 signalling pathway. Representative Western blot analysis of STAT3 and phospho-STAT3, proteins in MIA-PaCa-2 cells exposed to LIF (10 nM) alone or in combination with increasing concentration of mifepristone (10, 20, 50 µM) for 20 min. (F) Densitometric analysis demonstrating phospho-STAT3/STAT3 ratio.

Article Snippet: Protein extracts were electrophoresed on 12% acrylamide Tris-Glycine gel (Invitrogen), blotted to the nitrocellulose membrane, and then incubated overnight with primary Abs against STAT3 (sc-8019 1:500; Santa Cruz Biotechnology) and phosho-Stat3 (GTX118000 1:1000; Genetex).

Techniques: In Vitro, Migration, Expressing, Wound Healing Assay, Transferring, Generated, Incubation, Western Blot, Concentration Assay

Journal: bioRxiv

Article Title: Next generation sequencing analysis of gastric cancer identifies the leukemia inhibitory factor receptor (LIFR) as a driving factor in gastric cancer progression and as a predictor of poor prognosis

doi: 10.1101/2022.05.05.490785

Figure Lengend Snippet: Representative Western blot analysis of A) LIFR, JAK1 and phospho-JAK1, STAT3 and phospho-STAT3, proteins in MKN45 exposed to LIF (10 nM) alone or in combination with EC359 (25 nM and 100 nM) for 20 minutes. GAPDH was used as loading control. B) Densitometric analysis demonstrating LIFR expression, phospho-JAK1/JAK1 and phospho-STAT3/STAT3 ratio. The blot shown is representative of another one showing the same pattern.

Article Snippet: Protein extracts were electrophoresed on 12% acrylamide Tris-Glycine gel (Invitrogen), blotted to nitrocellulose membrane, and then incubated overnight with primary Abs against Jak1 (sc-7228 1:500; Santa Cruz Biotechnology), phospho-Jak1 (GTX25493 1:1000; Genetex), STAT3 (sc-8019 1:500; Santa Cruz Biotechnology), phosho-STAT3 (GTX118000 1:1000; Genetex), and GAPDH (bs2188R 1:1000; Bioss antibodies).

Techniques: Western Blot, Control, Expressing